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The Fimbrial Protein is a Virulence Factor and Potential Vaccine Antigen of Avibacterium paragallinarum.

Authors
  • Liu, C-C1
  • Ou, S-C1
  • Tan, D-H1
  • Hsieh, M-K1
  • Shien, J-H2
  • Chang, P-C1, 3
  • 1 A Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan. , (Taiwan)
  • 2 B Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan. , (Taiwan)
  • 3 C Agricultural Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan. , (Taiwan)
Type
Published Article
Journal
Avian Diseases
Publisher
BioOne (American Association of Avian Pathologists)
Publication Date
Sep 01, 2016
Volume
60
Issue
3
Pages
649–655
Identifiers
DOI: 10.1637/11410-031316-Reg.1
PMID: 27610725
Source
Medline
Keywords
License
Unknown

Abstract

Fimbriae are recognized as virulence factors and potential vaccine antigens of several pathogenic bacteria, but the function of the fimbriae from Avibacterium paragallinarum is not well known. In this study, a gene encoding the fimbrial protein FlfA was identified in A. paragallinarum . Sequencing analysis of the putative promoter region of flfA suggests that flfA expression in A. paragallinarum might be controlled by phase variation. The flfA gene from A. paragallinarum was expressed as a recombinant protein (r-FlfA) in Escherichia coli . Immunization with r-FlfA conferred chickens protection against challenge infection with A. paragallinarum . Virulence assays showed that the flfA-deficient mutants of A. paragallinarum were less virulent than their parental wild-type strains. These results indicated that the fimbrial protein FlfA is a virulence factor and potential vaccine antigen from A. paragallinarum .

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