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Dissociation of store release from transmembrane influx of calcium in human neutrophils

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
313
Issue
2
Identifiers
DOI: 10.1016/0014-5793(92)81426-m
Keywords
  • Neutrophil
  • Ca2+Store
  • Ca2+Cloud
  • Ratio Imaging

Abstract

Abstract Release of Ca 2+ from intracellular stores was visualised in individual neutrophils in the presence of the Mn 2+ or SKF 96365, Influx of Mn 2+ quenched fura-2 close to the plasma membrane but did not quench fura-2 at the site of store release. The size and location of the ‘cloud’ of elevated Ca 2+ was unaffected by the channel blocker SKF 96365. Furthermore, the size and location was unaffected by the presence of extracellular Ca 2+. This dissociation of transmembrane influx from store release demonstrates that the entry of Ca 2+ into the cytosol of neutrophils occurs directly into the cytosol and not via the store site.

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