Affordable Access

Publisher Website

Efflux of cyclic guanosine 3′,5′-monophosphate from cerebellar slices stimulated byl-glutamate or high K+or N-methyl-N′-nitro-N-nitrosoguanidine

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
68
Issue
1
Identifiers
DOI: 10.1016/0304-3940(86)90236-3
Keywords
  • Cyclic Guanosine 3′
  • 5′-Monophosphate Synthesis
  • Cyclic Guanosine 3′
  • 5′-Monophosphate Efflux
  • Probenecid
  • Nitroso Compound
  • L-Glutamate
  • Depolarization

Abstract

Abstract The efflux of cyclic guanosine 3′,5′-monophosphate (cGMP) was studied from rat cerebellar slices which were stimulated by l-glutamate (1 mM), or by depolarizing concentrations of K + (60 mM) or by the nitroso compound, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). Via different mechanisms these agents stimulated cGMP synthesis producing several fold elevation of the cGMP content of the tissue slices. Simultaneously with the elevation of the intracellular (intra slice) concentrations of cGMP, a temperature- and time-dependent efflux of cGMP into the medium took place. This efflux was maximal at 5 min and could be inhibited by the known inhibitor of anion transport, probenecid, in a concentration-dependent manner. The results suggest the presence of an efflux system for cGMP, which may participate together with the well-characterized 3′,5′-cyclic nucleotide phosphodiesterases in reduction of elevated cGMP levels.

There are no comments yet on this publication. Be the first to share your thoughts.