Affordable Access

The location of the active-site histidine residue in the primary sequence of papain

Authors
Publication Date
Source
PMC
Keywords
  • Articles

Abstract

Papain that had been irreversibly inhibited with 1,3-dibromo[2-14C]acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and α-chymotrypsin the radioactive peptides were purified chromatographically. Their amino acid composition indicated that cysteine-25 and histidine-106 were cross-linked. Since cysteine-25 is known to be the active-site cysteine residue, histidine-106 must be the active-site histidine residue.

There are no comments yet on this publication. Be the first to share your thoughts.