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Fetal bovine serum-free culture of endothelial progenitor cells-progress and challenges.

Authors
  • Bauman, E1, 2, 3
  • Granja, P L1, 2, 3, 4
  • Barrias, C C1, 2, 4
  • 1 Instituto de Inovação e Investigação em Saúde (i3S), Universidade do Porto, Porto, Portugal. , (Portugal)
  • 2 Instituto de Engenharia Biomédica (INEB), Universidade do Porto, Porto, Portugal. , (Portugal)
  • 3 Faculdade de Engenharia da Universidade do Porto (FEUP), Porto, Portugal. , (Portugal)
  • 4 Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal. , (Portugal)
Type
Published Article
Journal
Journal of Tissue Engineering and Regenerative Medicine
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jul 01, 2018
Volume
12
Issue
7
Pages
1567–1578
Identifiers
DOI: 10.1002/term.2678
PMID: 29701896
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Two decades after the first report on endothelial progenitor cells (EPC), their key role in postnatal vasculogenesis and vascular repair is well established. The therapeutic potential of EPC and their growing use in clinical trials calls for the development of more robust, reproducible, and safer methods for the in vitro expansion and maintenance of these cells. Despite many limitations associated with its usage, fetal bovine serum (FBS) is still widely applied as a cell culture supplement. Although different approaches aiming at establishing FBS-free culture have been developed for many cell types, adequate solutions for endothelial cells, and for EPC in particular, are still scarce, possibly due to the multiple challenges that have to be faced when culturing these cells. In this review, we provide a brief overview on the therapeutic relevance of EPC and critically analyse the available literature on FBS-free endothelial cell culture methods, including xeno-free, serum-free, and chemically defined systems. Copyright © 2018 John Wiley & Sons, Ltd.

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