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Null Mutation of the dam or seqA Gene Suppresses Temperature-Sensitive Lethality but Not Hypersensitivity to Novobiocin of muk Null Mutants

American Society for Microbiology
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  • Genetics And Molecular Biology


Escherichia coli mukF, mukE, and mukB null mutants have common phenotypes such as temperature-dependent colony formation, anucleate cell production, chromosome cutting by septum closure, and abnormal localization of SeqA-DNA clusters. We show here that the associated muk null mutations cause hypersensitivity to novobiocin. Null mutation of either dam or seqA suppressed partially the temperature-sensitive lethality but failed to suppress the anucleate cell production and the hypersensitivity to novobiocin caused by muk null mutations.

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