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Steric considerations regarding the biodegradation of cholesterol to pregnenolone.-exclusion of (22S)-22-hydroxycholesterol and 22-ketocholesterol as intermediates

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
94
Issue
2
Identifiers
DOI: 10.1016/0006-291x(80)91266-8

Abstract

Abstract (22 S )-[22- 3H 1]-Cholesterol was incubated with an adrenocortical preparation and the isolated (22 R )-[22- 3H 1]-22-hydroxycholesterol had a small loss of radioactivity, proving that direct replacement of the hydrogen from the now hydroxylated position occurred. In addition [1- 3H 1]-4-methylpentanol was isolated, which also had incurred a relatively small loss of its specific activity, thereby excluding (20 R )-3β,20-dihydroxycholest-5-en-22-one as an important metabolite in the degradation of cholesterol to pregnenolone by adrenal tissue.

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