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Feasibility of real-time polymerase chain reaction in whole blood to identifyStreptococcus pneumoniaein patients with community-acquired pneumonia

Journal
Diagnostic Microbiology and Infectious Disease
Publisher
Elsevier
Publication Date
Jan 01, 2008
Volume
61
Issue
1
Identifiers
DOI: 10.1016/j.diagmicrobio.2007.12.011
Keywords
License
Unknown

Abstract

Abstract We assessed a real-time quantitative polymerase chain reaction (PCR) assay targeting the lytA and ply gene of Streptococcus pneumoniae. Both assays were applied to whole blood samples from 28 adult patients with community-acquired pneumonia. Our findings suggest the lytA PCR is more sensitive, and the quantitative aspect of the assay shows promise as an aid to clinical judgment.

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