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Improved procedures for purification of human and canine creatine kinase isoenzymes

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
123
Identifiers
DOI: 10.1016/0009-8981(82)90114-0
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract Improved, and relatively simple procedures are described for the purification of human and canine creatine kinase isoenzymes with much higher yields (30 to 60%) and purity (450 kU/g) than previously possible. Contaminant albumin was removed from the MB preparation by albumin antibodies attached to Sepharose-4B. All isoenzyme preparations exhibited a single protein band on SDS gel electrophoresis. Using specific antisera to the isoenzymes and albumin the preparations were shown to be immunochemically pure and to give reliable results in a radioimmunoassay.

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