Affordable Access

Publisher Website

Techniques of obtaining and preparing the porcine liver for experimental and clinical temporary ex vivo perfusion

Authors
Journal
Journal of Surgical Research
0022-4804
Publisher
Elsevier
Publication Date
Volume
6
Issue
3
Identifiers
DOI: 10.1016/s0022-4804(66)80064-1
Disciplines
  • Medicine

Abstract

Summary Methods have been presented of porcine preoperative maintenance, induction and maintenance of anesthesia, intraoperative heparinization and hemodilution, prophylactic buffering prior to and during surgery along with the details of hepatectomy including hazards and rationales of the phases involved. These techniques have been utilized in 30 experimental preparations for isolated ex vivo perfusion studies and in 5 clinical perfusions. In the latter situations wherein the human-ex vivo perfused porcine liver is utilized as a possible therapeutic adjunct in the treatment of terminal hepatic failure, the avoidance of the initial phase of vasoconstriction secondary to an increase in transhepatic vascular resistance 3,4 in the excised liver becomes of paramount importance in maintaining adequate flow rates to support prolonged ex vivo porcine hepatic viability.

There are no comments yet on this publication. Be the first to share your thoughts.