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Basic Fibroblast Growth Factor Synthesis by Human Peritoneal Mesothelial Cells:Induction by Interleukin-1

American Journal Of Pathology
Publication Date
DOI: 10.1016/s0002-9440(10)65516-2
  • Bfgf Synthesis By Peritoneal Mesothelium
  • Chemistry
  • Medicine


Peritoneal mesothelial cells are uniquely located to regulate cellular events in the peritoneal cavity and are an important source for various cytokines and growth factors. This study was conducted to analyze the capacity of human peritoneal mesothelial cells (HPMCs) to synthesize and release basic fibroblast growth factor (bFGF) and to characterize its regulation by inflammatory cytokines. HPMCs constitutively synthesized and released considerable amounts of bFGF as detected by a specific immunoassay. Almost 80% of bFGF (1547 ± 173 pg/10 5 cells) was localized intracellularly. Approximately 20% of the bFGF (357 ± 27 pg/10 5 cells) was associated with extracellular matrix components on the HPMC surface. Small amounts of bFGF (<1%) were detectable in tissue culture supernatants (8.4 ± 1.4 pg/10 5 cells). Treatment of HPMCs with interleukin-1β (IL-1β; 1 ng/ml) resulted in a significant increase in bFGF production. The intracellular bFGF content showed a rapid but only transient increase, which was significant above background levels after 24 hours (41% increase; P < 0.05). This increase in intracellular bFGF concentration was associated with an induction of the release of bFGF. Within 96 hours, the release of bFGF to the cell surface and into the supernatant increased by 58% (564 ± 52.4 pg/10 5 cells; P < 0.01) and by 214% (26.4 ± 3.2 pg/10 5 cells; P < 0.001), respectively. Neither tumor necrosis factor-α nor interferon-γ affected bFGF synthesis by HPMCs. Stimulation of HPMCs with IL-1β increased steady-state levels of bFGF-specific mRNA. Immunohistochemical analyses of peritoneal tissue revealed constitutive expression of bFGF by HPMCs. This in situ expression proved to be most pronounced in areas of serosal inflammation in activated HPMCs. Our study demonstrates that HPMCs synthesize and release significant amounts of bFGF and that the expression of this growth factor is significantly up-regulated by the proinflammatory cytokine IL-1β. The data support the view that HPMCs are key regulators of abdominal disease processes such as peritonitis, peritoneal fibrosis, or peritoneal tumor metastasis.

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