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Protoplast-Derived Plants in Nicotiana plumbaginifolia Viviani: Improving the Regeneration Response of Wild Type and Mutant Cultures

Authors
Journal
Journal of Plant Physiology
0176-1617
Publisher
Elsevier
Publication Date
Volume
119
Issue
5
Identifiers
DOI: 10.1016/s0176-1617(85)80009-2
Keywords
  • Nicotiana Plumbaginifolia
  • Biochemical Mutants
  • Hormones
  • Protoplasts
  • Regeneration
Disciplines
  • Medicine

Abstract

Summary Nicotiana plumbaginifolia is considered a good plant for studies of somatic cell genetics and development of genetic manipulation procedures. Substantial improvements of the plant regeneration capacity in this species which is a good source of protoplasts was obtained by (1) developing a general scheme of protoplast processing that suits the requirements of both advanced cell technology (selection of mutants, fusion of protoplasts, genetic transformation) and totipotency, and (2) modifying the regeneration medium with respect to sugar, hormone, and major mineral content. An optimal sequence of culture steps was established which allows us to produce fertile plants from mutant cell lines, revertants, fusion products among metabolically deficient mutants, and DNA-treated cultures within three months of culturing.

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