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Effect of cyclic AMP and cyclic GMP on the autophosphorylation of elongation factor 1 from wheat embryos

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
128
Issue
1
Identifiers
DOI: 10.1016/0006-291x(85)91643-2
Disciplines
  • Biology

Abstract

Abstract Extensively purified EF-1 H (EF-1αββ′γ) from wheat embryos had a protein kinase activity and phosphorylated EF-1β which is one of the two EF-Ts-like factors (EF-1β and EF-1β′). In this reaction ATP and GTP were equally effective as phosphate donors, and threonine residue was phosphorylated. At 10 −7M,3′,5′ cyclic GMP stimulated both the phosphorylation and phe-tRNA binding reactions, whereas 3′,5′ cyclic AMP inhibited both reactions. These findings indicate that phosphorylation of EF-1 H may play an important role in the translational control of protein biosynthesis at the elongation step.

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