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Systematic profiling of polyclonal HIV antibodies and prediction of effector functions

Authors
Journal
Retrovirology
1742-4690
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
9
Identifiers
DOI: 10.1186/1742-4690-9-s2-p86
Keywords
  • Poster Presentation
Disciplines
  • Biology

Abstract

Systematic profiling of polyclonal HIV antibodies and prediction of effector functions POSTER PRESENTATION Open Access Systematic profiling of polyclonal HIV antibodies and prediction of effector functions E Brown From AIDS Vaccine 2012 Boston, MA, USA. 9-12 September 2012 Background Antibody-dependent effector functions (ADEF) with the ability to recruit the innate immune response may play an important role for the spread of HIV infections. ADEF are mediated by the antibody Fc and depend on antibody class and glycosylation status. We describe the systematic profiling of the Fc regions of HIV-specific antibodies isolated from different patient sera. Methods A LuminexTM-based suspension array was used to cap- ture HIV antibody fractions binding to various HIV anti- gens on beads and probe them for binding to (1) antibody class-specific binding reagents, (2) Fc receptors, (3) com- plement proteins and (4) different lectins. The obtained binding profiles are correlated with other measures of effector function and may help to understand which ADEF are crucial to provide protection. Results Subclassing data has been used to correlate patients with enhanced measures of effector function such as phagocytosis or ADCVI. In addition, results of the Fc- gamma-receptor binding assay have been correlated to traditional measures of affinity such as SPR, showing that our method is giving useful results. We have also used epitope-specific reagents like the resurfaced stabi- lized core form of gp120 to isolate and probe eppitope- specific antibodies. Conclusion We hope to use our system as a means to quickly evalu- ate antibodies induced in an HIV vaccine setting. Being able to get a rapid profile of the polyclonal antibody response should be a useful predictor of vaccine efficacy. In addition, the assay we have devised is easily customiz- able, as antigens and receptors can be tailored to fit our interests. Published: 13 September 2012 doi:10.1186/1742-4690-9-S2-P86 Cite this article

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