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Phospholipid vesicle fusion monitored by fluorescence energy transfer

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
88
Issue
1
Identifiers
DOI: 10.1016/0006-291x(79)91707-8

Abstract

Abstract A method is presented which allows the observation of phospholipid vesicle fusion by the occurrence of Förster resonance energy transfer between the amphiphilic probes dansyldipalmitoylphosphatidylethanolamine and 3-[4-( p -N,N-didecylaminostyryl)-1-pyridinium]-propylsulfonate. This method is applied to the Ca ++ mediated fusion of phosphatidyl serine vesicles.

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