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Fast-heating mass spectrometry of phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, and sphingomyelin

Authors
  • Dessort, Daniel
  • Mersel, Marcel
  • Lepage, Pierre
  • Van Dorsselaer, Alain
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier BV
Publication Date
Jan 01, 1984
Volume
142
Issue
1
Pages
43–52
Identifiers
DOI: 10.1016/0003-2697(84)90514-1
Source
Elsevier
Keywords
License
Unknown

Abstract

A fast-heating probe and chemical ionization have been used to obtain mass spectra of the synthetic glycerophospholipids 1,2-diacyl- sn-glycero-3-phosphocholine, 1,2-diacyl- sn-glycero-3-phosphoethanolamine, and 1-monoacyl-2-lyso- sn-glycero-3-phosphocholine. The phospholipids investigated gave quasimolecular peaks and fragment ions, with preferential cleavage of the CO bond (β position to the phosphorus atom) and loss of phosphoethanolamine or phosphocholine. This technique makes possible the analysis of mixtures of intact phosphatidylethanolamine, phosphatidylcholine, 2-lysophosphatidylcholine, and sphingomyelin isolated from natural sources such as egg yolk or brain. Only minor and inexpensive modifications of a standard mass spectrometer are required.

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