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Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography.

Authors
  • Pazourek, Jiří1
  • 1 Department of Chemical Drugs, University of Veterinary and Pharmaceutical Science, Palackého 1/3, CZ-611 42 Brno, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Carbohydrate research
Publication Date
Jun 04, 2014
Volume
391
Pages
55–60
Identifiers
DOI: 10.1016/j.carres.2014.03.010
PMID: 24785388
Source
Medline
Keywords
License
Unknown

Abstract

A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, d-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo- and d-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12min at 10°C, though higher temperatures (25°C or 40°C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9mg/L at 10°C, and 5mg/L at 25°C, resp.

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