Affordable Access

Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.

Authors
  • Piyawattanametha, Wibool
  • Barretto, Robert P J
  • Ko, Tony H
  • Flusberg, Benjamin A
  • Cocker, Eric D
  • Ra, Hyejun
  • Lee, Daesung
  • Solgaard, Olav
  • Schnitzer, Mark J
Type
Published Article
Journal
Optics letters
Publication Date
Jul 01, 2006
Volume
31
Issue
13
Pages
2018–2020
Identifiers
PMID: 16770418
Source
Medline
License
Unknown

Abstract

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.

Report this publication

Statistics

Seen <100 times