Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.
- Published Article
- Publication Date
Jul 01, 2006
Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/16770418