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Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam.

Authors
  • Bianchini, P
  • Diaspro, A
Type
Published Article
Journal
Journal of microscopy
Publication Date
Mar 01, 2012
Volume
245
Issue
3
Pages
225–228
Identifiers
DOI: 10.1111/j.1365-2818.2011.03577.x
PMID: 22171566
Source
Medline
License
Unknown

Abstract

In this paper we report stimulated emission depletion (STED) and two-photon excitation (2PE) fluorescence microscopy with continuous wave (CW) laser beam using a new generation laser scanning confocal microscope equipped for STED-CW (TCS STED-CW, Leica Microsystems, Mannheim, Germany). We show the possibility to achieve CW-2PE with the very same beam used for STED-CW. This feature extends the performance of the microscope allowing multimodal imaging (CW-2PE, STED-CW, confocal).

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