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A fast fluorescence imaging flow cytometer for phytoplankton analysis.

Authors
Type
Published Article
Journal
Optics Express
1094-4087
Publisher
The Optical Society
Publication Date
Volume
21
Issue
20
Pages
23921–23926
Identifiers
DOI: 10.1364/OE.21.023921
PMID: 24104302
Source
Medline
License
Unknown

Abstract

We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 ± 0.06 μm for a wide size range ~1 μm to ~200 μm. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.

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