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Fast and direct quantification of adrenal steroids by tandem mass spectrometry in serum and dried blood spots.

Authors
Type
Published Article
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
861
Issue
1
Pages
117–122
Identifiers
PMID: 18053779
Source
Medline
License
Unknown

Abstract

We present a fast and reproducible method for steroid analysis (corticosterone, deoxycorticosterone, progesterone, 17alpha-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, testosterone, dihydrotestosterone and cortisol) in small volumes of serum and in dried blood spot samples by LC-MS/MS. No derivatisation was needed. LC separation was achieved by using an Atlantis C18 column and water-methanol-formic acid gradient as a mobile phase and a flow rate of 250 microL/min over a run time of 6 min. Steroids were measured in MRM mode with electrospray interface (positive ion mode). Validation showed excellent precision, sensitivity, recovery and linearity with coefficients of determination r2>0.992.

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