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Far-red/near-infrared emitting, two-photon absorbing, and bio-stable amino-Si-pyronin dyes

Authors
  • Kim, Kyeong Hwan
  • Singha, Subhankar
  • Jun, Yong Woong
  • Reo, Ye Jin
  • Kim, Hye Rim
  • Ryu, Hye Gun
  • Bhunia, Snehasis
  • Ahn, Kyo Han
Publication Date
Oct 01, 2019
Source
[email protected]
Keywords
License
Unknown
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Abstract

Organic fluorophores emitting in the far-red/near-infrared (NIR) wavelength region are in great demand for minimal autofluorescence and reduced light scattering in deep tissue or whole body imaging. Currently, only a few classes of far-red/NIR fluorophores are available including widely used cyanine dyes, which are susceptible to photobleaching and form nonfluorescent aggregates. Even rare are those far-red/NIR emitting dyes that have two-photon imaging capability. Here we report a new class of far-red/NIR-emitting dyes that are photo-stable, very bright, biocompatible, and also two-photon absorbing. The introduction of an electron-withdrawing group such as N-acyl or N-alkoxycarbonyl groups on the C-10-amino substituent of the new julolidine-derived amino-Si-pyronin dyes (ASiP(j)), which emit in the far-red region, causes large bathochromic shifts, leading to NIR-emitting amino-Si-pyronin dyes (NIR-ASiP(j)) having high cellular stability. Furthermore, the ASiP(j)-NIR-ASiP(j) couple offers a novel ratiometric bioimaging platform with a large spectral gap, as demonstrated here with a boronate-containing NIR-ASiP(j) derivative that is converted to the corresponding ASiP(j) dye upon reaction with hydrogen peroxide. / 1 / N

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