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Far-Red Fluorescent Lipid-Polymer Probes for an Efficient Labeling of Enveloped Viruses.

Authors
  • Lacour, William1, 2
  • Adjili, Salim1, 2
  • Blaising, Julie3
  • Favier, Arnaud1, 2
  • Monier, Karine1
  • Mezhoud, Sarra2
  • Ladavière, Catherine2
  • Place, Christophe1, 4
  • Pécheur, Eve-Isabelle3
  • Charreyre, Marie-Thérèse1, 2
  • 1 Université Lyon, Ens de Lyon, CNRS, Laboratoire Joliot-Curie, F-69342, Lyon, France. , (France)
  • 2 Université Lyon, INSA Lyon, Université Claude Bernard, CNRS, Laboratoire Ingénierie des Matériaux Polymères, F-69621, Villeurbanne, France. , (France)
  • 3 Université Lyon, Univ Claude Bernard, INSERM U1052, CNRS 5286, Centre de Recherche en Cancérologie de Lyon (CRCL), F-69424, Lyon, France. , (France)
  • 4 Université Lyon, Ens de Lyon, Université Claude Bernard, CNRS, Laboratoire de Physique, F-69342, Lyon, France. , (France)
Type
Published Article
Journal
Advanced healthcare materials
Publication Date
Aug 01, 2016
Volume
5
Issue
16
Pages
2032–2044
Identifiers
DOI: 10.1002/adhm.201600091
PMID: 27113918
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Far-red emitting fluorescent lipid probes are desirable to label enveloped viruses, for their efficient tracking by optical microscopy inside autofluorescent cells. Most used probes are rapidly released from membranes, leading to fluorescence signal decay and loss of contrast. Here, water-soluble lipid-polymer probes are synthesized harboring hydrophilic or hydrophobic far-red emitting dyes, and exhibiting enhanced brightness. They efficiently label Hepatitis C Virus pseudotyped particles (HCVpp), more stably and reproducibly than commercial probes, and a strong fluorescence signal is observed with a high contrast. Labeling with such probes do not alter virion morphology, integrity, nor infectivity. Finally, it is shown by fluorescence microscopy that these probes enable efficient tracking of labeled HCVpp inside hepatocarcinoma cells used as model hepatocytes, in spite of their autofluorescence up to 700 nm. These novel fluorescent lipid-polymer probes should therefore enable a better characterization of early stages of infection of autofluorescent cells by enveloped viruses. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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