Affordable Access

Failure to detect hydrogen-sulphide production in lactose/sucrose-fermenting Enterobacteriaceae, using triple sugar iron agar.

Authors
  • Kolmos, H J
  • Schmidt, J
Type
Published Article
Journal
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
Publication Date
Feb 01, 1987
Volume
95
Issue
1
Pages
85–87
Identifiers
PMID: 3551500
Source
Medline
License
Unknown

Abstract

Triple Sugar Iron agar failed to detect hydrogen sulphide in 44 out of 69 hydrogen-sulphide producing strains of Enterobacteriaceae, which at the same time fermented lactose and/or sucrose. The species involved were Salmonella typhi, Salmonella enteritidis, Citrobacter freundii, Escherichia coli, and Proteus vulgaris. By contrast, no false-negative reactions were observed in 74 strains, which fermented neither lactose nor sucrose. Failure to detect hydrogen sulphide was probably due to acidification of the medium following the fermentation of carbohydrates. A medium without carbohydrates is preferable in diagnostic situations where hydrogen-sulphide detection is of great importance.

Report this publication

Statistics

Seen <100 times