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Factors required for calcium dependent acetylcholine release from isolated torpedo synaptic vesicles

Authors
  • Michaelson, Daniel M.
  • Pinchasi, Irit
  • Sokolovsky, Mordechai
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Publication Date
Jan 01, 1978
Volume
80
Issue
3
Pages
547–552
Identifiers
DOI: 10.1016/0006-291X(78)91603-0
Source
Elsevier
Keywords
License
Unknown

Abstract

The release of acetylcholine (Ach) from Torpedo synaptic vesicles has been investigated. Factors have been found which induce Ca +2 dependent Ach release from the synaptic vesicles. In the absence of these factors, the vesicles are not affected by Ca +2. Addition of a soluble factor to the vesicles induces a Ca +2-dependent release of their Ach. This secretion is enhanced by a non-vesicular membranous component which, by itself, does not induce the Ca +2-dependent release. These results demonstrate that vesicular Ach release may be studied in vitro and thus will enable the study, at the molecular level, of the biochemical events underlying neurotransmission.

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