Abstract 1. 1. The responsiveness of dystrophic avian muscle to acetylcholine may be altered due to reported elevated acetylcholinesterase activity. 2. 2. To test this hypothesis, the responsiveness of normal and dystrophic muscle in vivo to intra-arterial injection of acetylcholine, carbamylcholine and d-tubocurarine was compared. 3. 3. Results showed that dystrophic muscle was less responsive to acetylcholine, more responsive d-tubocurarine and equally responsive to carbamylcholine when compared to normal suggesting enhanced acetylcholine hydrolysis occurs in vivo in dystrophic avian muscle.