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Elimination of autosampler carryover in a bioanalytical HPLC-MS/MS method: a case study

Authors
Journal
Journal of Pharmaceutical and Biomedical Analysis
0731-7085
Publisher
Elsevier
Publication Date
Volume
36
Issue
5
Identifiers
DOI: 10.1016/j.jpba.2004.09.010
Keywords
  • Bioanalytical Methods
  • Hplc/Ms
  • Autosampler Carryover

Abstract

Abstract A case study in identifying and eliminating the source of autosampler carryover in a bioanalytical HPLC-MS/MS assay is described. Through a series of systematic experiments, the carryover was traced to the injection valve and was eliminated by switching from a partial loop to a full loop injection, which provided more effective flushing of the sample flow path. The susceptibility of the HPLC system to carryover was demonstrated to depend on the absolute sensitivity of the detection method and the mass of analyte injected at the assay lower limit of quantitation (LLOQ).

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