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Cell-to-cell variability of gene expression dynamics in inducible regulatory networks

Authors
Publisher
Ludwig-Maximilians-Universität München
Publication Date
Keywords
  • Fakultät Für Physik
Disciplines
  • Biology

Abstract

Cell-to-cell variability of gene expression dynamics in inducible regulatory networks Cell-to-cell variability of gene expression dynamics in inducible regulatory networks Judith Megerle Mu¨nchen 2011 Cell-to-cell variability of gene expression dynamics in inducible regulatory networks Judith Megerle Dissertation an der Fakulta¨t fu¨r Physik der Ludwig–Maximilians–Universita¨t Mu¨nchen vorgelegt von Judith Megerle aus Regensburg Mu¨nchen, den 25. Januar 2011 Erstgutachter: Prof. Dr. Joachim Ra¨dler Zweitgutachter: Prof. Dr. Ulrich Gerland Tag der mu¨ndlichen Pru¨fung: 2. Ma¨rz 2011 Contents Zusammenfassung 1 Summary 3 1 Introduction 5 2 Basic concepts 9 2.1 Gene expression and transcriptional regulation . . . . . . . . . . . . . . . . 9 2.2 Gene regulatory networks . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.3 Stochasticity in gene expression . . . . . . . . . . . . . . . . . . . . . . . . 10 2.4 Noise: Use or nuisance? . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.5 Modeling gene expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 2.6 Fluorescent proteins as reporters for gene expression . . . . . . . . . . . . . 17 3 Quantitative time-lapse fluorescence microscopy 19 3.1 Fluorescence microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 3.2 Sample environment and flow system . . . . . . . . . . . . . . . . . . . . . 21 3.3 Image analysis of time-lapse movies . . . . . . . . . . . . . . . . . . . . . . 23 3.4 Fluorescence signal and bacterial autofluorescence . . . . . . . . . . . . . . 29 4 Determination of the GFP maturation time on the single cell level 31 5 Timing and dynamics of gene expression in the arabinose system 33 5.1 The arabinose utilization system . . . . . . . . . . . . . . . . . . . . . . . . 34 5.2 Heterogeneous timing of single cell gene induction . . . . . . . . . . . . . . 37 5.2.1 Single cell induction kinetics

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