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Metabolic studies of rat liver plasma membranes usingd-[l-14C]glucosamine

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
0005-2736
Publisher
Elsevier
Publication Date
Volume
225
Issue
2
Identifiers
DOI: 10.1016/0005-2736(71)90216-1
Disciplines
  • Biology

Abstract

Abstract The metabolism of plama membranes of rat liver cells was studied using d-[ l- 14C]glucosamine. The labelling of plasma membranes occurred more slowly than that of microsomes, reaching a maximum at about 3 h after injection compared to 1.5 h for microsomes, and the radioactivity decayed with a half-life of 37 h which is close to the value obtained using [ guanidino- 14C]arginine to label proteins. Hexosamine and sialic acid of plasma membranes were found to metabolize at practically equal rates. These findings suggest that complete glycoproteins are built into plasma membranes after being synthesized in the endoplasmic reticulum, and the polypeptide and carbohydrate moieties then turn over in a concerted manner.

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