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Identification of a potent synthetic HIV1 immunogen compromising gag-P24 tandem T- and B-cell epitopes

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
264
Issue
2
Identifiers
DOI: 10.1016/0014-5793(90)80255-h
Keywords
  • Acquired Immunodeficiency Syndrome
  • Vaccine Design
  • Antigenic Epitope
Disciplines
  • Chemistry
  • Computer Science

Abstract

Abstract Recent studies indicate that the gag gene products may play a crucial role in the immune response against HIV infection since clinical progression to AIDS is associated with a reduction in the level of circulating antibodies to gag p 24 and antibodies raised against p 17 peptide can inhibit HIV1 infection in vitro. Using conventional structure prediction algorithms for T-cell and B-cell epitopes, we have selected and chemically synthesized several gag peptides. In particular, an unconjugated HIV1-p 24 peptide containing both B- and T-cell epitopes in tandem plus Freund's adjuvant induced a strong antibody response in both mice and rabbits against p 24 and its precursor p 55 as judged by immunoblotting. In addition, the peptide presented in the appropriate MHC context was shown to be highly stimulatory for p 24 specific murine T-cell clones.

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