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Structural studies of a mitochondria-free plasma membrane fraction from rat liver

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
0005-2736
Publisher
Elsevier
Publication Date
Volume
352
Issue
1
Identifiers
DOI: 10.1016/0005-2736(74)90178-3
Disciplines
  • Biology

Abstract

Abstract Liver plasma membranes virtually free of contaminating mitochondria have been prepared. Sodium dodecylsulfate-polyacrylamide gel electrophoresis reveals a membrane protein resistant to papain digestion in the intact membranes but readily hydrolyzed in membranes disrupted by detergent or sonication. Electron microscopy of mechanically deformed membranes reveals fibrils within the membrane which appear to be protein in nature but which also persist in papain digested membranes.

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