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A novel denaturation of DNA

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
0005-2787
Publisher
Elsevier
Publication Date
Volume
353
Issue
2
Identifiers
DOI: 10.1016/0005-2787(74)90184-1
Disciplines
  • Physics

Abstract

Abstract Calf thymus DNA is found to be completely denatured and lose all secondary structure in a solvent system which contains roughly equal volumes of methanol and ethanol, and a buffer concentration below 15%. The phenomenon is invesigated by monitoring the ultraviolet circular dichroism and absorption of the system. The results contrast with those obtained in similar organic solvent systems where DNA was found to maintain a secondary structure. No rationalization of these results can be given at this time.

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