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Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis

Authors
Journal
Comparative Immunology Microbiology and Infectious Diseases
0147-9571
Publisher
Elsevier
Publication Date
Volume
33
Issue
5
Identifiers
DOI: 10.1016/j.cimid.2009.01.001
Keywords
  • Leptospirosis
  • Mat
  • Elisa
  • Hap1
  • Lipl32
  • Peptide
  • Leptospirose
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Leptospirosis is a worldwide zoonosis. Today, serological diagnosis is generally assessed by MAT. We performed ELISA with a synthetic peptide derived from Hap1/lipL32 which is a protein expressed only by pathogenic Leptospira. Repeatability and thresholds were defined with 85 controls sera and 119 hospitalized leptospirosis. The PP-ELISA repeatability and IgM/IgG cut-off values were based on control sera. For these cut-off values, we observed the IgM-PP-ELISA specificity of 89%, whereas it was 100% for the IgG. Then, we compared PP-ELISA and standard MAT results for leptospirosis patients. The concordance rate for IgM-PP-ELISA and MAT was low (43%), whereas it was 85% for IgG-PP-ELISA and MAT. During the first 5 days after hospitalization, PP-ELISA gave positive results in 13 out of 16 patients (81%) whereas 8 out of 14 patients (57%) were positive to MAT. ELISA using Hap1/lipL 32-derived synthetic peptide PP is an earlier serological diagnosis of human leptospirosis than MAT.

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