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A new procedure for the determination of hemoglobin

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
26
Issue
1
Identifiers
DOI: 10.1016/0009-8981(69)90288-5

Abstract

Abstract A new and simple procedure for the determination of hemoglobin iron is presented. The procedure involves separation of the iron from hemoglobin by 2.5% sodium hypochlorite solution and measurement of the absorbance of the Fe 2+-chelate formed with a new ligand, 7-bromo-1,3-dihydro-1-(3-dimethylaminopropyl)-5-(2-pyridyl)-2 H-1,4-benzodiazepin-2-one dihydrochloride. A comparison of the results obtained by the present procedure and the hemoglobincyanide method show excellent agreement.

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