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Species Pattern and Phylogenetic Relationships of Trichoderma Strains in Rice Fields of Southern Caspian Sea, Iran

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Akadémiai Kiadó
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DOI: doi: 10.1556/crc.39.2011.4.11

Abstract

Species pattern and phylogenetic relationships of <Emphasis Type="Italic">Trichoderma</Emphasis> strains in rice fields of Southern Caspian Sea, Iran Species Pattern and Phylogenetic Relationships of Trichoderma Strains in Rice Fields of Southern Caspian Sea, Iran S. NAEIMI1, S.A. KHODAPARAST2, M. JAVAN-NIKKHAH3, C. VÁGVÖLGYI4 and L. KREDICS4* 1Iranian Research Institute of Plant Protection, Tehran, P.O. Box 19395-1454, Iran 2Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht 41635-1314, P.O. Box 41889, Iran 3Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj 3158711167, Iran 4Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary (Received 15 December 2010; accepted 3 March 2011) As a first step of a project aimed at the identification of potential biocontrol agents of Rhizoctonia solani, the rice sheath blight fungus, we surveyed the biodiversity of the genus Trichoderma based on sequence of the internal transcribed spacer (ITS) 1 and 2 of the ribo- somal RNA gene cluster in paddy fields in Mazandaran province, Northern Iran. Amongst the six obtained species of Trichoderma, T. harzianum and T. virens proved to be the most fre- quent species in this habitat. Sequence alignment and phylogenetic analysis revealed that the T. harzianum isolates can be divided into 14 different ITS genotypes clustering in four groups. Our results are in agreement with previous molecular studies, which also revealed that T. harzianum is a complex species comprising more or less different ITS genotypes. T. virens was not as diverse as T. harzianum and three different genotypes were distinguished which constituted only one cluster. All T. atroviride and T. hamatum strains had identical ITS se- quences. Keywords: Trichoderma, T. harzianum, rice, paddy fields, biodiversity, ITS, phylogeny Introduction Sheath blight caused by Rhizoctonia solani is one of the most serious rice diseases world- wide

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