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Bioethanol production from the nutrient stress-induced microalgaChlorella vulgarisby enzymatic hydrolysis and immobilized yeast fermentation

Authors
Journal
Bioresource Technology
0960-8524
Publisher
Elsevier
Volume
153
Identifiers
DOI: 10.1016/j.biortech.2013.11.059
Keywords
  • Bioethanol
  • Microalgae
  • Chlorella Vulgaris
  • Enzymatic Saccharification
  • Nitrogen Stress
Disciplines
  • Biology

Abstract

Abstract The microalga Chlorella vulgaris is a potential feedstock for bioenergy due to its rapid growth, carbon dioxide fixation efficiency, and high accumulation of lipids and carbohydrates. In particular, the carbohydrates in microalgae make them a candidate for bioethanol feedstock. In this study, nutrient stress cultivation was employed to enhance the carbohydrate content of C. vulgaris. Nitrogen limitation increased the carbohydrate content to 22.4% from the normal content of 16.0% on dry weight basis. In addition, several pretreatment methods and enzymes were investigated to increase saccharification yields. Bead-beating pretreatment increased hydrolysis by 25% compared with the processes lacking pretreatment. In the enzymatic hydrolysis process, the pectinase enzyme group was superior for releasing fermentable sugars from carbohydrates in microalgae. In particular, pectinase from Aspergillus aculeatus displayed a 79% saccharification yield after 72h at 50°C. Using continuous immobilized yeast fermentation, microalgal hydrolysate was converted into ethanol at a yield of 89%.

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