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Regulation of MeCP2 induced heterochromatin remodeling

Authors
Publisher
Ludwig-Maximilians-Universität München
Publication Date
Keywords
  • Fakultät Für Biologie
Disciplines
  • Biology

Abstract

Regulation of MeCP2 induced heterochromatin remodeling Regulation of MeCP2 induced heterochromatin remodeling Annette Becker München 2010 Regulation of MeCP2 induced heterochromatin remodeling Annette Becker Dissertation an der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Diplom-Biochemikerin Annette Becker aus Köln München, den 20. Juli 2010 Erstgutachter: Prof. Dr. Heinrich Leonhardt Zweitgutachter: Prof. Dr. Ruth Brack-Werner Tag der mündlichen Prüfung: 21.10.2010 CONTENT 1 1 SUMMARY 3 1.1 Zusammenfassung 3 1.2 Summary 5 2 INTRODUCTION 7 2.1 DNA methylation 7 2.2 Recognition of methylated DNA by methyl-CpG binding domain proteins 8 2.2.1 Methyl-CpG binding domain protein 1 (MBD1) 8 2.2.2 Methyl-CpG binding domain protein 2 (MBD2) 10 2.2.3 Methyl-CpG binding domain protein 3 (MBD3) 12 2.2.4 Methyl-CpG binding domain protein 4 (MBD4) 12 2.2.5 MeCP2 14 2.3 Biological functions of MeCP2 19 2.3.1 Binding of MeCP2 to DNA in vitro 19 2.3.2 Binding of MeCP2 to chromatin in vitro 20 2.3.3 MeCP2 DNA binding and effect on chromatin architecture in vivo 20 2.3.4 Interacting partners of MeCP2 22 2.3.5 Post-translational modifications of MeCP2 modulate its functions 24 2.3.6 Poly(ADP-ribose)polymerase-1 25 3 QUESTIONS AND AIMS OF THIS WORK 27 4 METHODS AND MATERIALS 28 4.1 Molecular biology methods 28 4.1.1 Construction of expression plasmids 28 4.2 Cell biology methods 29 4.2.1 Cell culture and transfection 29 4.2.2 ImmunoFISH 30 4.2.3 Microscopy, image analysis and statistical evaluation 31 4.3 Biochemic

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