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A simple and sensitive bioassay for the detection of human interleukin-18/interferon-γ-inducing factor using human myelomonocytic KG-1 cells

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
209
Issue
2
Identifiers
DOI: 10.1016/s0022-1759(97)00164-6
Keywords
  • Il-18
  • Bioassay
  • Cytokine
  • Quantification
Disciplines
  • Medicine

Abstract

Abstract Interleukin-18 (IL-18)/interferon- γ-inducing factor (IGIF) is a novel cytokine which plays an important role in Th1 responses. Here we describe a simple, sensitive bioassay for human IL-18 using the human myelomonocytic cell line, KG-1, which produces IFN- γ in response to human IL-18. IFN- γ production induced by human IL-18 was completely blocked by an antibody against human IL-18. Human IL-18 could be measured in a concentration range from approximately 100 to 10,000 pg/ml, and intra- and inter-assay coefficient variations were both below 15%. It was possible to measure human IL-18 in human serum, cell lysate or culture supernatant by this bioassay. Thus, the human IL-18 bioassay can be expected to be useful in the investigation of the relationship between human IL-18 and various diseases or in analyzing the mechanisms of human IL-18 secretion from IL-18 producing cells.

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