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The mechanism of complement fixation by rabbit F(ab′)2: Properties of the bactericidal reaction

Authors
Journal
Immunochemistry
0019-2791
Publisher
Elsevier
Publication Date
Volume
14
Issue
5
Identifiers
DOI: 10.1016/0019-2791(77)90230-0

Abstract

Abstract The properties of the complement (C)-dependent bactericidal reaction of F(ab′) 2 antibodies to V. cholerae are described. The killing effect (a) is about 10 times less efficient on a weight basis than the killing mediated by the parent IgG molecule, (b) is abolished by prior heating of C to 56°C for 30 min, (c) cannot be ascribed to contamination with intact or partially degraded IgG, (d) is almost completely abolished by reduction and alkylation, (e) requires that both of the Fab′ subunits in F(ab′) 2 are specific, (f) is, in contrast to IgG, more sensitive to C dilution, and (g) appears to be more dependent on the O-antigen composition of the V. cholerae test strain than the parent IgG molecule.

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