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Quantitative analyses of matrix metalloproteinase activity after traumatic brain injury in adult rats

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
1280
Identifiers
DOI: 10.1016/j.brainres.2009.05.040
Keywords
  • Head Injury
  • Mmp-9
  • Cortex
  • Immunohistochemistry
  • Quantitative Real-Time Pcr
Disciplines
  • Biology
  • Chemistry
  • Design

Abstract

Abstract Recent laboratory evidence implicates matrix metalloproteinases (MMPs) as playing a pivotal role in ischemic and traumatic brain injuries (TBI). Here, quantitative real-time PCR analyses revealed that brains from TBI rats displayed significantly elevated MMP-9 expression at 24 h post-TBI, which remained upregulated at least until 48 h after injury. Immunohistochemical analyses similarly revealed significantly increased MMP-9 immunoreactivity at 24 and 48 h post-TBI. These results demonstrate that alterations in MMPs (i.e., MMP-9) commenced immediately after TBI, suggesting that treatment strategies designed to maintain MMP integrity should be initiated in the acute phase of injury.

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