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Improved rapid method for production and purification ofClostridium perfringenstype A enterotoxin

Authors
Journal
Journal of Microbiological Methods
0167-7012
Publisher
Elsevier
Publication Date
Volume
20
Issue
2
Identifiers
DOI: 10.1016/0167-7012(94)90011-6
Keywords
  • Clostridium Perfringens
  • Enterotoxin
  • Sporulation

Abstract

Abstract Clostridium perfringens strain FD-1041 was grown in Duncan-Strong medium containing raffinose for 5 h at 43°C. Enterotoxin in the cell extract was purified 5.5-fold by a modified ammonium sulfate-gel filtration method using Sephacryl S-200. The enterotoxin could be purified to homogeneity with 50% recovery in less than 48 h.

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