Altered metabolic requirements in cancer cell migration and metastasis

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Altered metabolic requirements in cancer cell migration and metastasis

Authors
Publisher
BioMed Central
Volume
6
Identifiers
DOI: 10.1186/1753-6561-6-s3-p32
Keywords
  • Poster Presentation

Abstract

Altered metabolic requirements in cancer cell migration and metastasis POSTER PRESENTATION Open Access Altered metabolic requirements in cancer cell migration and metastasis Jaewon Lee1,2*, M Rosa Ng1, Kerstin W Sinkevicius3,4,5, Carla F Kim3,4,5, Gaudenz Danuser1, Joan S Brugge1, Marcia C Haigis1,2 From Metabolism, diet and disease Washington, DC, USA. 29-31 May 2012 Background Metastasis poses a great challenge in clinical manage- ment of many cancers. Metabolic demands of cancer cell proliferation – i.e. elevated aerobic glycolysis for biomass generation – have been well characterized, but the contribution of altered metabolism to metastasis remains to be elucidated. While elevated aerobic glyco- lysis, a phenomenon termed Warburg effect, is a hall- mark of proliferative tumor cells, emerging evidence suggests that metastatic cancer cells have an opposite phenotype. Materials and methods We investigated the metabolism in cell lines of different metastatic capacity. In addition, to quantitatively study cell migration and metabolism, we used MCF10A breast epithelial cells with fluorescent histone tags along with live cell imaging and tracking by a custom MATLAB program to measure speed and behavior of migration. Results Our preliminary data suggest that the more metastatic cancer cells depend on mitochondrial metabolism. Furthermore, changing mitochondrial metabolism in MCF10A breast epithelial cell line affected not only the speed but also the pattern of cell migration. Consistent with the altered migratory behaviors, stimulation of mito- chondrial metabolism changed cell adhesion markers. Conclusions In sum, we show evidence that mitochondrial metabo- lism plays an important role in promoting cell migration and altering cell adhesion with implications for cancer metastasis. Author details 1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. 2The Paul F. Glenn Labs for the Biological Mechanisms of Aging, Harvard Medical School, Boston, MA 02115,

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