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Small-Angle X-ray Scattering Studies of Peptide–Lipid Interactions Using the Mouse Paneth Cell α-Defensin Cryptdin-4-Chapter Four

Elsevier Science & Technology
Publication Date
DOI: 10.1016/b978-0-12-381268-1.00016-1
  • Biology
  • Communication


Abstract In the presence of specialized proteins or peptides, a biological membrane can spontaneously restructure itself to allow communication between the intracellular and the extracellular sides. Examples of these proteins include cell-penetrating peptides and antimicrobial peptides (AMPs), which interact with cell membranes in complex ways. We briefly review cell-penetrating peptides and AMPs, and describe in detail how recombinant AMPs are made and their activity evaluated, using α-defensins as a specific example. We also review X-ray scattering methods used in studying peptide–membrane interactions, focusing on the procedures for small-angle X-ray scattering experiments on peptide–membrane interactions at realistic solution conditions, using both laboratory and synchrotron sources.

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