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Preparation and use of125I-labeled synthetic human (15-Leu)gastrin 1-17-I for radioimmunoassay of conventional human gastrins

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
87
Issue
1
Identifiers
DOI: 10.1016/0003-2697(78)90570-5

Abstract

Abstract This report describes a method of radiodination and use of synthetic human (15-Leu)gastrin in radioimmunoassay for synthetic or naturally occurring human gastrin. Specific activity of the [ 125I]monoiodo(15-Leu)gastrin was 45% higher than that obtained with conventional gastrin. Radioimmunoassay using a radio-labeled (15-Leu)gastrin trace was reliable between 10 −12 and 10 −9 molar concentrations of synthetic human or naturally occurring gastrin.

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