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Expression of canine interferon-β by a recombinant vaccinia virus

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
466
Issue
1
Identifiers
DOI: 10.1016/s0014-5793(99)01785-8
Keywords
  • Interferon-β
  • Vaccinia Virus
  • N-Linked Glycosylation
  • Antiviral Activity

Abstract

Abstract A recombinant vaccinia virus expressing canine interferon (IFN)-β was constructed (vv/cIFN-β). In rabbit kidney (RK13) and canine A72 cells infected with vv/cIFN-β, the recombinant canine IFN-β was detected in both cell extracts and supernatants, and the IFN activities of the culture supernatants were also detected. Inhibition of N-linked glycosylation by tunicamycin treatment indicated that the recombinant canine IFN-β was modified by N-linked glycosylation in a different way between RK13 and A72 cells, and that N-linked glycosylation is essential for its secretion. The growth of vv/cIFN-β at a low multiplicity of infection was inhibited by antiviral activity of canine IFN-β, indicating that this recombinant virus could be used as a suicide viral vector.

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