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A temperature profile in batch culture to increase the production of the recombinant UDP-glucuronosyltransferase 2B4 inEscherichia coli

Authors
Journal
Process Biochemistry
1359-5113
Publisher
Elsevier
Publication Date
Volume
31
Issue
3
Identifiers
DOI: 10.1016/0032-9592(95)00055-0
Disciplines
  • Biology

Abstract

Abstract A cDNA encoding human liver UDP-glucuronosyltransferase 2B4, which glucuronidates the bile acid hyodeoxycholic acid, has been expressed in Escherichia coli as a fusion protein with Staphylococcus aureus protein A. The expression of this protein, under the control of the thermo-inducible λ-P R promoter, was induced by a shift in culture temperature. In order to increase both the biomass and the amount of recombinant protein, the temperature profile in the batch culture and especially the influence of the time of induction have been studied. A lag phase of 35°C, followed by exponential and stationary phases of 42 and 28°C respectively, allowed an increase of about 27% of UGT2B4 per litre of culture compared to the typical 28–42°C late induced batch culture.

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