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T-helper 1 and 2 serum cytokine assay in chronic opioid addicts.

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  • Rc0321 Neuroscience. Biological Psychiatry. Neuropsychiatry

Abstract

þÿ Eur Cytokine Netw. ٢٠٠٧ Dec;١٨(٤):٢١٠-٤. Epub ٢٠٠٧ Nov ١٢. T-helper ١ and ٢ serum cytokine assay in chronic opioid addicts. Azarang A, Mahmoodi M, Rajabalian S, Shekari MA, Nosratabadi J, Rezaei N. Source Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran. Abstract There are a few studies with conflicting results on the effects of opioids on the functioning of immune system. This study was performed to investigate the in vitro production of interferon- gamma and interleukin-١٠ after antigenic stimulation of cells using whole blood from opioid addicts. Blood samples were taken from ٢٠ chronically opioid-addicted persons, who voluntarily enrolled for detoxification (١٠ opium and ١٠ heroin addicts). Blood samples were also taken from ١٠ healthy individuals with no history of drug abuse as the control. Cell culture was performed in a whole blood culture assay. Diluted blood samples were stimulated with phytohemagglutinin or with lipopolysaccharide and the supernatants were collected to measure cytokineproduction. The results demonstrated a significant decrease in interferon- gamma production and an increase in interleukin-١٠ secretion in heroinaddicts, relative to the control group (٣٥٫٩+/-٢٦٫٣ versus ١١٠.٢+/-٦٠٫٣ pg/mL, p<٠٫٠١ and ٧١٫٨+/-٢٨٫٤ versus ١٧.١+/- ١٣٫٥ pg/mL, p<٠٫٠١, respectively), however the changes in these values in opium addicts were not significant compared to healthy individuals. The results could suggest that opioid addiction leads to a shift in the Th١/Th٢ cytokine balance of peripheral CD٤+ cells towards the Th٢ response, and opioid addictsdemonstrate reduced mitogenic responsiveness of lymphocytes relative to healthy individuals. PMID: ١٧٩٩٣٤٥٢ [PubMed - indexed for MEDLINE]

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