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Displaced granule cells in the molecular layer of the cerebellar cortex in mice treated with methylazoxymethanol

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
358
Issue
2
Identifiers
DOI: 10.1016/j.neulet.2003.12.108
Keywords
  • Development
  • Malformation
  • Green Fluorescent Protein
  • γ-Aminobutyric Acid
  • Purkinje Cells
  • Basket Cells
Disciplines
  • Biology
  • Medicine

Abstract

Abstract To study the effect of a reduced granule cell number on the development of the cerebellum, mice were administered with methylazoxymethanol (MAM) at birth and examined histologically at 21 days of age. In these mice, green fluorescent protein had been knocked in within the glutamic acid decarboxylase 67 gene, resulting in fluorescent GABAergic neurons. In severe cases, granule cells were greatly reduced in number and mixed with Purkinje cells instead of forming layers. In about 10% of the MAM-treated mice, an ectopic cell layer consisting of granule cells was observed within the molecular layer. Concomitantly, basket cells disappeared. The transient interruption of granule cell production in the external granular layer presumably resulted in their incomplete migration through the molecular layer.

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