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Assay for Hageman factor (factor XII) in human plasma by means of chromogenic substrate for plasma kallikrein

Authors
Journal
Thrombosis Research
0049-3848
Publisher
Elsevier
Publication Date
Volume
31
Issue
6
Identifiers
DOI: 10.1016/0049-3848(83)90118-4
Keywords
  • Chromogenic Substrate
  • Factor Xii
  • Kallikrein Activity
Disciplines
  • Biology

Abstract

Abstract Determination of factor XII was performed in a test system where plasma samples were diluted (1/400-1/3200) in buffer and factor XII deficient plasma. Diluted Cephotest R was used as activator and after 10 min activation time chromogenic substrate H-D-Pro-Phe-Arg-pNA was added. The assay was adopted to an enzyme analyzer. The reproducibility of the method was acceptable with a coefficient of variation of 7.8% in the normal range. The correlation of this method to a one-stage clotting assay for factor XII was good, r 0.79. The preliminary reference interval was calculated to be 52–147% of normal (mean+ 2 S.D.).

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