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Relationships between glycollate and formate metabolism in greening barley leaves

Authors
Journal
Phytochemistry
0031-9422
Publisher
Elsevier
Publication Date
Volume
21
Issue
7
Identifiers
DOI: 10.1016/s0031-9422(82)85003-6
Keywords
  • Hordeum Vulgare
  • Gramineae
  • Barley Leaves
  • Greening
  • Enzymes Of Glycollate And Formate Metabolism.
Disciplines
  • Biology

Abstract

Abstract The activities of enzymes catalysing glycollate oxidation, formate production and folate-dependent formate utilization were examined in the primary leaves of Hordeum vulgare cv Galt. Seedlings were grown for 6 days in darkness and then transferred to continuous light (500 μinsteins/m 2 per sec) for up to 5 days. Cell-free extracts of the primary leaves contained glycollate oxidase (EC 1.1.3.1), 10-formyltetrahydrofolate synthetase (EC 6.3.4.3), 5, 10-methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) and ability to enzymically decarboxylate glyoxylate. These activities increased during greening and at the end of the light treatment were 70–450% higher than etiolated controls. Greened primary leaves also incorporated [ 14C]formate at rates that were three- to four-fold higher than shown by etiolated leaves. The specific activity of 10-formyltetrahydrofolate synthetase was decreased by 20–35% when the leaves were greened in the presence of 10 mM hydroxysulphonate. This inhibitor also reduced the incorporation of [ 14C]formate by up to 45%. A potential flow of carbon from glycollate to 10-formyltetrahydrofolate via glyoxylate and formate was suggested by the data.

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